Anti-FLAG (DYKDDDDK) Tag agarose Beads were used to immunoprecipitate FLAG-tagged fusion proteins from HEK293T cell lysates. During elution with Peptide elution buffer, the enriched FLAG fusion protein are released from the agarose beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: FLAG fusion protein.
Anti-FLAG (DYKDDDDK) Tag agarose Beads
Anti-FLAG (DYKDDDDK) Tag agarose Beads
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Immunogen | Synthetic Peptide |
| Isotype | IgG |
| Application | IP, Protein purification |
| Reactivity | Species Independent |
| Conjugation | Agarose beads |
| Storage Buffer | 50% slurry in PBS pH7.4, 0.05% Proclin 300. Ensure thorough mixing before each use. Wide-mouth pipette tips are recommended. In case of difficulty in aspiration due to improper operation, additional Storage Buffer can be added at your discretion. |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Anti-FLAG (DYKDDDDK) Tag agarose Beads is an anti-FLAG Rabbit Recombinant monoclonal antibody covalently conjugated to agarose beads. It can be widely used for immunoprecipitation or purification of fusion proteins or their protein complexes with Flagger tags.
Picture
Picture
SDS-PAGE
