Enrich or deplete F4/80+ cells from spleen cells of C57BL/6 mice. Stain F4/80+ cells with FITC Rat anti-mouse F4/80 (S-R537) (SDT Cat: S0B5113), and gate on the live cell population for analysis.
Anti-F4/80 Nanobeads, mouse（RUO）
Anti-F4/80 Nanobeads, mouse（RUO）
Price:
Regular price
$535 USD
Regular price
Sale price
$535 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Format | Dextran-coated iron oxide nanomagnets conjugated with an anti-mouse F4/80 monoclonal antibody, in a PBS buffer system containing BSA and Poloxamer 188. |
| Capacity |
2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, allowing up to 200 sorting runs 1 mL magnetic beads: suitable for a total cell count of 1 × 10⁹, allowing up to 100 sorting runs |
| Principle of Separation | Using Anti-F4/80 Nanobeads, mouse anti‑F4/80+Cells are subjected to magnetic labeling. Subsequently, the cell suspension is loaded into a separation column positioned within the sorter's magnetic field. The magnetically labeled F4/80…+Cells remain retained within the column, while unlabeled cells elute; this fraction constitutes the F4/80-depleted fraction.+The negative fraction following cell sorting. After removing the sorting column from the magnetic field, the F4/80‑positive cells that were magnetically captured…+Cells can be eluted to obtain the positive‑selection fraction. If higher purity is required, it contains F4/80.+The positively sorted cell fraction must be subjected to a second round of sorting on a second column. |
| Reactivity | Mouse |
| Magnetic Bead Size | 20 nm |
| Separation Method | Column-Based |
| Selection Strategy | Positive Selection |
| Stability & Storage | Store protected from light at 2–8°C; do not freeze. |
Background
F4/80 is a member of the epidermal growth factor (EGF) transmembrane 7 (TM7) family, consisting of a seven-transmembrane molecule and a large extracellular domain containing multiple EGF-like modules. F4/80 is considered one of the most specific cell surface markers of murine macrophages. Constitutive high expression of F4/80 is observed on the surface of most tissue-resident macrophages, including splenic macrophages, microglia in the brain, Kupffer cells in the liver, and Langerhans cells in the skin. Furthermore, F4/80 expression is also regulated according to the physiological state of the cells.
Picture
Picture
Validation Data
