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Anti-Cetuximab Rabbit Polyclonal Antibody

Anti-Cetuximab Rabbit Polyclonal Antibody

Catalog Number: S0B6174 Application: ELISA Brand: Starter
Price:
Regular price $330 USD
Regular price Sale price $330 USD
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Product Details

Product Specification


Host Rabbit
Antibody Type Polyclonal antibody
Isotype IgG
Application ELISA
Purification Immunogen Affinity
Concentration 1 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS pH7.4

Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Background

The anti-Cetuximab antibody is a monoclonal or polyclonal antibody that specifically recognizes and targets Cetuximab. It is primarily used in scientific research, clinical testing, and drug monitoring. Cetuximab is a chimeric monoclonal antibody drug targeting the Epidermal Growth Factor Receptor (EGFR), widely used in the treatment of various malignant tumors such as colorectal cancer and head and neck squamous cell carcinoma. By binding to specific epitopes of Cetuximab, the Anti-Cetuximab antibody can be employed in experimental techniques like Enzyme-Linked Immunosorbent Assay (ELISA), Western Blot, and Immunohistochemistry (IHC) to quantify Cetuximab concentration in biological samples, analyze its pharmacokinetic properties, or assess the potential development of anti-drug antibodies (ADA) in patients. Furthermore, this antibody aids in researching resistance mechanisms to Cetuximab, such as monitoring changes in the EGFR signaling pathway or the efficiency of drug-receptor binding, thereby providing a critical tool for optimizing targeted therapy regimens.

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ELISA

Antigen (Cetuximab, human total IgG) 1μg/ml is coated overnight at 4°C. Anti- Cetuximab Rabbit polyclonal Antibody is added in serial dilutions and incubated at 37°C for 1 hours. Peroxidase Goat Anti-Rabbit IgG (min X Hu Sr Prot) secondary antibody is added at 37°C for 40 min. Substrate solution is added and developed for 5 minutes at room temperature. Absorbance is measured at 450 nm.