Antigen (Cetuximab, human total IgG) 1μg/ml is coated overnight at 4°C. Anti- Cetuximab Rabbit polyclonal Antibody is added in serial dilutions and incubated at 37°C for 1 hours. Peroxidase Goat Anti-Rabbit IgG (min X Hu Sr Prot) secondary antibody is added at 37°C for 40 min. Substrate solution is added and developed for 5 minutes at room temperature. Absorbance is measured at 450 nm.
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | ELISA |
| Purification | Immunogen Affinity |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
The anti-Cetuximab antibody is a monoclonal or polyclonal antibody that specifically recognizes and targets Cetuximab. It is primarily used in scientific research, clinical testing, and drug monitoring. Cetuximab is a chimeric monoclonal antibody drug targeting the Epidermal Growth Factor Receptor (EGFR), widely used in the treatment of various malignant tumors such as colorectal cancer and head and neck squamous cell carcinoma. By binding to specific epitopes of Cetuximab, the Anti-Cetuximab antibody can be employed in experimental techniques like Enzyme-Linked Immunosorbent Assay (ELISA), Western Blot, and Immunohistochemistry (IHC) to quantify Cetuximab concentration in biological samples, analyze its pharmacokinetic properties, or assess the potential development of anti-drug antibodies (ADA) in patients. Furthermore, this antibody aids in researching resistance mechanisms to Cetuximab, such as monitoring changes in the EGFR signaling pathway or the efficiency of drug-receptor binding, thereby providing a critical tool for optimizing targeted therapy regimens.
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ELISA
