ICC shows positive staining in HeLa cells. Tubulin-biotin antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Biotin Recombinant Rabbit mAb (PE Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Anti-Biotin Recombinant Rabbit mAb (PE Conjugate) (S-3083)
Anti-Biotin Recombinant Rabbit mAb (PE Conjugate) (S-3083)
Price:
Regular price
$185 USD
Regular price
Sale price
$185 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Biotin |
| Clone Number | S-3083 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:500 | Hu, Ms, Rt |
Background
As a labeling agent, the core value of Biotin lies in exploiting the extraordinarily strong non-covalent binding between biotin and avidin or streptavidin (with dissociation constants on the order of 10⁻¹⁵ M): biotin is covalently conjugated to antibodies, nucleic acid probes, or other target molecules, and detection or capture is then achieved using avidin/streptavidin conjugated to enzymes, fluorophores, or colloidal gold, thereby enabling signal amplification and specific tracing. Because the biotin molecule is very small (approximately 244 Da), labeling usually does not significantly interfere with the biological activity or binding capacity of the target molecule. This system is widely used in enzyme-linked immunosorbent assays, immunoblotting, immunohistochemistry, and lateral flow strip tests: in nucleic acid detection strips, for example, amplification products are labeled with both biotin and fluorescein—biotin immobilizes the product on the streptavidin test line, while fluorescein binds colorimetric particles to generate a visible signal
Picture
Picture
Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells. Tubulin-biotin antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Biotin Recombinant Rabbit mAb (PE Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Tubulin-biotin antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Biotin Recombinant Rabbit mAb (PE Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
