Flow cytometric analysis of CD73 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes was stained with PE Rat Anti- Mouse CD3 Antibody and either Biotin Rat IgG Isotype Control (left panel) or SDT Biotin Rat Anti-Mouse CD73 Antibody (right panel) at 1 μg/test followed by stained with Biotin Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) at 1/10000 dilution. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-3083)
Biotin Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-3083)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Biotin |
| Clone Number | S-3083 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, ICFCM, FCM |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:500 | Species Independent |
| ICFCM | 1:5000-1:10000 | Species Independent |
| FCM | 1:5000-1:10000 | Species Independent |
Background
Biotin, also known as vitamin B7 or vitamin H, is a water-soluble B-complex vitamin that serves as a crucial coenzyme for carboxylases, facilitating key biochemical processes such as gluconeogenesis, fatty acid synthesis, and amino acid metabolism. Since the human body cannot synthesize biotin on its own, it must be obtained through dietary sources (e.g., egg yolks, nuts, and animal liver) or in small amounts via gut microbiota. Its molecular structure consists of a ureido ring fused with a tetrahydrothiophene ring, and the valeric acid side chain enables binding to enzyme proteins for biological activity. Although widely believed to strengthen hair and nails due to its affinity for keratin, scientific evidence primarily supports biotin supplementation for addressing deficiencies or metabolic disorders, which may manifest as dermatitis, hair loss, or neurological abnormalities. The recommended daily intake for adults is approximately 30 μg, with higher doses needed for pregnant women or specific populations. While biotin toxicity is rare, excessive intake can interfere with laboratory tests (e.g., thyroid hormones, cardiac biomarkers).
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Immunocytochemistry
ICC shows positive staining in HeLa cells. Tubulin-biotin antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Biotin Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in NIH/3T3 cells. Tubulin-biotin antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Biotin Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Tubulin-biotin antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Biotin Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
