Negative cells were labeled using Anti-biotin Nanobeads and a Mouse T cell Biotin Antibody Cocktail to enrich T cells from mouse splenocytes. Subsequently, fluorescent staining was performed with anti-CD3 (clone: 145-2C11) Alexa Fluor® 488, and the live cell population was gated for analysis.
Anti-biotin Nanobeads
Anti-biotin Nanobeads
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Product Details
Product Details
Product Specification
| Format | Dextran-coated iron tetroxide nanomagnetic beads coupled with mouse anti-biotin monoclonal antibody |
| Capacity |
2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations 1 mL magnetic beads: suitable for 5×108total cells, and can perform up to 50 separations |
| Principle of Separation | Stain the cells with biotinylated primary antibodies or ligands. Subsequently, magnetically label the cells using Anti-biotin Nanobeads. Then, load the cell suspension onto a separation column placed within the separator's magnetic field. The magnetically labeled cells are retained inside the column, while the unlabeled cells pass through. After removing the column from the magnetic field, the cells that were retained magnetically can be eluted as the positively selected cell fraction. |
| Reactivity | Human, Mouse |
| Separation Method | Column-Based |
| Selection Strategy | Positive Selection |
| Stability & Storage | Store protected from light at 2–8°C; do not freeze. |
Background
Anti-biotin Nanobeadis designed for the indirect magnetic labeling and separation of cells or other biological materials that have been labeled with biotinylated primary antibodies, ligands, or biotinylated antibody mixtures. The biotinylated molecules are recognized by anti-biotin monoclonal antibodies conjugated to the magnetic beads.
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Validation Data
