WB result of ANGPT2 Recombinant Rabbit mAb
Primary antibody: ANGPT2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HUVEC whole cell lysate 20 µg
Lane 3: TF-1 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 70 kDa
ANGPT2 Recombinant Rabbit mAb (S-1768-2)
ANGPT2 Recombinant Rabbit mAb (S-1768-2)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ANGPT2 |
| Synonyms | Angiopoietin-2; ANG-2 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | O15123 |
| Clone Number | S-1768-2 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, HUVEC, TF-1, HeLa, mouse spleen, rat kidney |
| Predicted Reactivity | Bv, Pg, Dg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Angiopoietin-2 (ANGPT2) is a member of the angiopoietin family and functions as a ligand for the TEK/TIE2 receptor, competing with angiopoietin-1 (ANGPT1) for binding sites. It plays a critical role in regulating angiogenesis and vascular remodeling. During embryonic development and tumorigenesis, ANGPT2 disrupts the vascular stability mediated by ANGPT1 and can induce endothelial cell apoptosis. Additionally, ANGPT2 is upregulated in various inflammatory diseases, where it contributes to the regulation of inflammation-related signaling pathways. In the tumor microenvironment, ANGPT2 synergizes with VEGF to promote endothelial cell migration and proliferation, acting as a pro-angiogenic signal. It also plays a role in lymphangiogenesis and serves as a prognostic biomarker in several cancers, including renal, liver, cervical, and gastric cancers.
Picture
Picture
Western Blot
WB result of ANGPT2 Recombinant Rabbit mAb
Primary antibody: ANGPT2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 70 kDa
WB result of ANGPT2 Recombinant Rabbit mAb
Primary antibody: ANGPT2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 70 kDa
