The ANDR(LBD) activity was detected using HTRF binding assay. The reaction was performed by incubating the ANDR(LBD) protein, CRBN/DDB1 and ARV-110, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Synonyms | DHTR, NR3C4, AR |
Accession | P10275-1 |
Amino Acid Sequence | D551-T919,N-terminal 8x His and SUMO tag, C-terminal Myc tag |
Expression System | E.coli |
Molecular Weight | 56.5 kDa |
Purity | >75% by SDS-PAGE |
Conjugation | Unconjugated |
Tag | His Tag, SUMO, Myc |
Physical Appearance | Liquid |
Storage Buffer | 25mM Hepes, 150 mM NaCl, 2 mM DTT, 10 μM DHT, 5% glycerol, pH7.5 |
Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg ANDR(LBD)
2 3 μg ANDR(LBD)
