IF shows positive staining in paraffin-embedded human colon cancer. Anti- AMACR p504s (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
AMACR/p504s Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-401-29)
AMACR/p504s Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-401-29)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | AMACR/P504S |
| Synonyms | Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion, Peroxisome |
| Accession | Q9UHK6 |
| Clone Number | SDT-401-29 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 555 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:100-1:200 | null |
Background
α-Methylacyl-CoA racemase (AMACR; P504S) catalyzes a key chiral inversion step in the metabolism of branched-chain fatty acids, ibuprofen and related drugs. Protein levels are increased in all prostate and some other cancer cells and it is used as a marker (P504S). The enzyme requires no cofactors and catalyzes its reaction by a stepwise 1,1-proton transfer via an enolate intermediate. The biological role of AMACR in cancer is complex, linking lipid metabolism with nuclear receptor (e.g. FXR and PPAR) activity and expression of enzymes such as cyclooxygenase-2 (COX-2). A number of rationally designed AMACR inhibitors have been reported in the literature as potential cancer treatments. The opportunities and challenges for development of acyl-CoA esters as inhibitors are discussed from a medicinal chemical viewpoint. Other challenges for drug development include the problems in assaying enzymatic activity and the prediction of structure-activity relationships (SAR). [PMID: 23376124]
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Immunofluorescence
IF shows positive staining in paraffin-embedded human prostatic cancer. Anti- AMACR p504s (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
