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ALPL His Tag Protein, Human

ALPL His Tag Protein, Human

Catalog Number: UA106010 Brand: UA BIOSCIENCE
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$170 USD
$170 USD
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Product Details

Product Specification


Species Human
Synonyms Alkaline phosphatase, tissue-nonspecific isozyme, AP-TNAP, TNAP, TNSALP, Alkaline phosphatase 2, Alkaline phosphatase liver/bone/kidney isozyme, Phosphoamidase, Phosphocreatine phosphatase, Akp-2, Akp2
Accession P09242
Amino Acid Sequence

TPhe18-Ser502, with C-His Tag
Expression System HEK293
Molecular Weight

Predicted MW: 55.3 kDa Observed MW: 65-90 kDa
Purity >95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

0.2M PBS, pH7.4 with 3% trehalose.
Reconstitution

Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

Background

Alkaline Phosphatase, Tissue-Nonspecific Isozyme (TNAP) is a ubiquitously expressed enzyme encoded by the ALPL gene. As the predominant alkaline phosphatase in humans, it is crucial for bone and tooth mineralization. TNAP hydrolyzes inorganic pyrophosphate, a potent mineralization inhibitor, and provides inorganic phosphate to promote hydroxyapatite crystal growth. Beyond its skeletal role, TNAP is also vital in neuronal development, vitamin B6 metabolism, and modulating intestinal inflammation. Mutations in ALPL cause hypophosphatasia, a metabolic bone disorder characterized by defective mineralization. Thus, TNAP serves as a central regulator of phosphate metabolism across multiple physiological systems.

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SDS-PAGE

2μg（R: reducing conditions）