ALPI Recombinant Rabbit mAb (S-3768-104)

Catalog Number: S0B60045 Application: WB, IHC-P Reactivity: Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	ALPI
Synonyms	Intestinal-type alkaline phosphatase; IAP; Intestinal alkaline phosphataseAlternative nameAlkaline phosphatase 3; Akp-3; Akp3
Immunogen	Synthetic Peptide
Location	Cell membrane
Accession	P24822
Clone Number	S-3768-104
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Ms, Rt
Positive Sample	mouse small intestine
Purification	Protein A
Concentration	0.25 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Ms, Rt
IHC-P	1:2500	Ms, Rt

Background

ALPI (intestinal alkaline phosphatase) protein is a homodimeric glycoprotein primarily expressed on the brush border of the small intestinal mucosa, encoded by a gene located on human chromosome 2q37.1, and belongs to an important member of the tissue-nonspecific alkaline phosphatase family. As a core component of the intestinal mucosal barrier, ALPI not only participates in the digestion and absorption of nutrients by hydrolyzing phosphate esters but, more critically, catalyzes the dephosphorylation of lipopolysaccharide (LPS) and endogenous nucleotides, thereby exerting endotoxin detoxification and anti-inflammatory effects, effectively inhibiting bacterial translocation and intestinal inflammation. At the molecular level, ALPI maintains the intestinal epithelial mechanical barrier by enhancing the expression of tight junction proteins (such as ZO-1 and Occludin) and regulates the TLR4/NF-κB signaling pathway to sustain immune homeostasis. Recent studies have also revealed that ALPI expression is regulated by various physiological and pathological conditions, such as complement C3 deficiency, and its upregulation can restore the expression of mucin-related genes and improve the constipation phenotype. Furthermore, mutations or aberrant expression of the ALPI gene are not only closely associated with inflammatory bowel disease and necrotizing enterocolitis but have also been found to affect glycolipid metabolism, suggesting that this protein plays a critical role in maintaining intestinal homeostasis and preventing metabolic diseases.

Picture

Western Blot

WB result of ALPI Recombinant Rabbit mAb
Primary antibody: ALPI Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse small intestine lysate 20 µg
Negative control: mouse kidney lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 80 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse small intestine. Anti-ALPI antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse colon. Anti-ALPI antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat small intestine. Anti-ALPI antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat colon. Anti-ALPI antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.