WB result of Alpha Tubulin Recombinant Mouse mAb
Primary antibody: Alpha Tubulin Recombinant Mouse mAb at 1/20000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: Hep G2 whole cell lysate 20 µg
Lane 4: HEK-293: whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 52 kDa
This blot was developed with high sensitivity substrate
Alpha Tubulin Recombinant Mouse mAb (S-5704)
Alpha Tubulin Recombinant Mouse mAb (S-5704)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Alpha Tubulin |
| Synonyms | Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P68363 |
| Clone Number | S-5704 |
| Antibody Type | Mouse mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Predicted Reactivity | Rb, Dg, Gt, Ck, Sh |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Alpha tubulin is a ubiquitously expressed cytoskeletal protein that polymerizes with beta-tubulin to form microtubules, thereby orchestrating mitotic spindle assembly, intracellular transport, cell shape and motility; its over-expression is frequently observed in multiple cancers where it drives proliferation, migration and invasion, correlates with advanced stage and poor survival, modulates innate and adaptive immune signaling, and predicts response to immune-checkpoint inhibition, making it an emerging prognostic biomarker and potential therapeutic target.
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Picture
Western Blot
WB result of Alpha Tubulin Recombinant Mouse mAb
Primary antibody: Alpha Tubulin Recombinant Mouse mAb at 1/20000 dilution
Lane 1:mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 52 kDa
WB result of Alpha Tubulin Recombinant Mouse mAb
Primary antibody: Alpha Tubulin Recombinant Mouse mAb at 1/20000 dilution
Lane 1:rat brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 52 kDa
This blot was developed with high sensitivity substrate
WB result of Alpha Tubulin Recombinant Mouse mAb
Primary antibody: Alpha Tubulin Recombinant Mouse mAb at 1/20000 dilution
Lane 1:COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 52 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Alpha Tubulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Alpha Tubulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Alpha Tubulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Alpha Tubulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain. Anti-Alpha Tubulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-Alpha Tubulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Alpha Tubulin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
