IF shows positive staining in paraffin-embedded human prostate. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
alpha smooth muscle Actin Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (S-132-63)
alpha smooth muscle Actin Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (S-132-63)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | SMA, Alpha-actin-2, ACTA2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P62736 |
| Clone Number | S-132-63 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:100 |
Background
α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas Sα-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.
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Immunofluorescence
IF shows positive staining in paraffin-embedded human stomach. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse colon. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat colon. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
