SDT-132_alpha smooth muscle Actin (Alexa Fluor® 647)_FC_1
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody at 1/200 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
alpha smooth muscle Actin Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (SDT-132-63)
alpha smooth muscle Actin Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (SDT-132-63)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | alpha smooth muscle Actin (SMA) |
| Synonyms | Alpha-actin-2,ACTA2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P62736 |
| Clone Number | SDT-132-63 |
| Antibody Type | Recombinant mAb |
| Application | ICC, ICFCM, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:1000 | null |
| ICFCM | 1:200 | null |
| ICC | 1:1000 | null |
Background
α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas α-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.
Picture
Picture
FC
Immunofluorescence
IF shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human leiomyosarcoma. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
