WB result of ALPG Rabbit pAb
Primary antibody: ALPG Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: PANC-1 whole cell lysate 20 µg
Low expression control: HeLa whole cell lysate; A549 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 65 kDa
ALPG Rabbit Polyclonal Antibody
ALPG Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ALPG |
| Synonyms | Alkaline phosphatase, germ cell type; ALP-1; Alkaline phosphatase Nagao isozyme; Alkaline phosphatase; placental-like; Germ cell alkaline phosphatase (GCAP); Placental alkaline phosphatase-like (PLAP-like); ALPPL; ALPPL2 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P10696 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | A431, PANC-1 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
ALPG, also known as ALPPL2 or germ-cell alkaline phosphatase, is a 532-amino-acid, GPI-anchored glycoprotein enzyme (EC 3.1.3.1) belonging to the alkaline phosphatase family that is normally restricted to testis, thymus and trophoblast but becomes aberrantly over-expressed on the surface of a broad spectrum of solid tumours—including seminoma, mesothelioma, ovarian, pancreatic and gastric cancers—where it catalyzes the hydrolysis of phosphate monoesters, contributes to tumour growth and invasion, and serves as both a cancer-specific therapeutic target and a cell-surface marker for naïve pluripotency in stem-cell biology.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human placenta. Anti-ALPG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-ALPG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human seminoma. Anti-ALPG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-ALPG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
