WB result of ALP Recombinant Rabbit mAb
Primary antibody: ALP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate
ALP Recombinant Rabbit mAb (S-2786-41)
ALP Recombinant Rabbit mAb (S-2786-41)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ALP |
| Synonyms | PDZ and LIM domain protein 3; Actinin-associated LIM protein; Alpha-actinin-2-associated LIM protein; PDLIM3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q53GG5 |
| Clone Number | S-2786-41 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | U-2 OS, mouse skeletal muscle, rat skeletal muscle, rat heart |
| Predicted Reactivity | Pg, Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms, Rt |
Background
Alkaline phosphatase (ALP) is a ubiquitous membrane-bound metalloenzyme that catalyzes the non-specific hydrolysis of phosphate monoesters at alkaline pH, existing as four distinct human isozymes—tissue-non-specific (liver/bone/kidney), intestinal, placental and germ-cell—whose differential glycosylation and post-translational modifications generate characteristic electrophoretic patterns exploited clinically to pinpoint the source of elevated serum activity that serves as a sensitive but non-specific biomarker for hepatobiliary obstruction, bone formation by osteoblasts, pregnancy, malignancy, or metabolic disorders, while intracellularly the enzyme participates in lipid transport, dephosphorylation of receptors, and modulation of inflammatory signaling through its ectophosphatase activity on extracellular ATP.
Picture
Picture
Western Blot
WB result of ALP Recombinant Rabbit mAb
Primary antibody: ALP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse skeletal muscle lysate 20 µg
Negative control: mouse kidney lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate
WB result of ALP Recombinant Rabbit mAb
Primary antibody: ALP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Lane 2: rat skeletal muscle lysate 20 µg
Lane 3: rat heart lysate 20 µg
Negative control: rat kidney lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate
