WB result of ALKBH5 Recombinant Rabbit mAb
Primary antibody: ALKBH5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 38-46 kDa
ALKBH5 Recombinant Rabbit mAb (S-2264-39)
ALKBH5 Recombinant Rabbit mAb (S-2264-39)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ALKBH5 |
| Synonyms | RNA demethylase ALKBH5; Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; ABH5; OFOXD1 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | Q6P6C2 |
| Clone Number | S-2264-39 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | MCF7, Jurkat, HeLa, HepG2 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000 | Hu |
| IHC-P | 1:250 | Hu |
Background
ALKBH5 is a nuclear 45-kDa Fe(II)- and 2-oxoglutarate-dependent dioxygenase that selectively demethylates N6-methyladenosine (m6A) in single-stranded RNA, thereby dynamically modulating mRNA splicing, export, stability, and translation; highly expressed in testes and within nuclear speckles, it is essential for spermatogenesis and fertility, while in somatic tissues it orchestrates glucose and lipid homeostasis through independent regulation of GCGR and mTORC1 signaling, and its dysregulation—via hypoxia-inducible upregulation or cancer-associated overexpression—drives stemness, proliferation, and chemoresistance in breast cancer, glioblastoma, and ovarian cancer by demethylating oncogenic transcripts such as NANOG and FOXM1, positioning ALKBH5 as a central epitranscriptomic hub linking metabolism, reproduction, and malignancy.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-ALKBH5 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-ALKBH5 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-ALKBH5 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-ALKBH5 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
