The ALK4 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the ALK4 protein, ATP and substrate at 25℃ for 60 min, and adding ADP-Glo reagent at 25℃ for 40 min, then reading RLU with BMG.
Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P36896-1 |
| Amino Acid Sequence |
N150-I505 NYHQRVYHNRQRLDMEDPSCEMCLSKDKTLQDLVYDLSTSGSGSGLPLFVQRTVARTIVLQEIIGKGRFGEVWRGRWRGGDVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTIEGMIKLALSAASGLAHLHMEIVGTQGKPGIAHRDLKSKNILVKKNGMCAIADLGLAVRHDAVTDTIDIAPNQRVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNSGGVHEEYQLPYYDLVPSDPSIEEMRKVVCDQKLRPNIPNWWQSYEALRVMGKMMRECWYANGAARLTALRIKKTLSQLSVQEDVKI |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 67.4 kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 20 mM reduced glutathione, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH 7.5 |
| Stability & Storage |
Stable for
12 months upon stored at -80℃ from the date of receipt. And avoid repeated
freeze-thaws cycles.
|
Picture
Picture
Bioactivity


SDS-PAGE

SEC-HPLC

