WB result of Alix Rabbit pAb
Primary antibody: Alix Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 90-100 kDa
Alix Rabbit Polyclonal Antibody
Alix Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Alix |
| Synonyms | Programmed cell death 6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95; AIP1; ALIX; KIAA1375; PDCD6IP |
| Immunogen | Recombinant Protein |
| Location | Secreted, Cytoplasm, Melanosome |
| Accession | Q8WUM4 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, A549, PC-3, MCF7, NIH/3T3, mouse brain, C6, rat brain, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
Alix, also known as ALG-2-interacting protein X or PDCD6IP, is a crucial multifunctional adaptor protein belonging to the Bro1 domain-containing family that plays a pivotal role in regulating endosomal sorting and membrane trafficking processes, particularly within the Endosomal Sorting Complex Required for Transport (ESCRT) pathway. Structurally characterized by an N-terminal Bro1 domain, a central proline-rich region, and a C-terminal V domain, Alix serves as a molecular bridge that interacts with various ESCRT-III components, such as CHMP4, and ubiquitinated cargo proteins to facilitate the formation of intraluminal vesicles in multivesicular bodies, thereby directing cellular proteins toward lysosomal degradation or exosomal release. Beyond its canonical function in membrane remodeling and cytokinesis, Alix is extensively involved in apoptotic signaling through its interaction with apoptosis-linked gene 2 (ALG-2) and participates in viral budding mechanisms, making it a significant subject of study in understanding cellular homeostasis, neurodegenerative diseases, and cancer progression.
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Western Blot
WB result of Alix Rabbit pAb
Primary antibody: Alix Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 90-100 kDa
WB result of Alix Rabbit pAb
Primary antibody: Alix Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 90-100 kDa
WB result of Alix Rabbit pAb
Primary antibody: Alix Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 90-100 kDa
