| Flow cytometric analysis of Mouse Ly-6G/Ly-6C (Gr-1) expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Brilliant Violet 421™ Rat Anti-Mouse CD11b Antibody and either Alexa Fluor® 700 Rat IgG2b, κ Isotype Control (Left panel) or SDT Alexa Fluor® 700 Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody (Right panel) at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software. |
Alexa Fluor® 700 Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody (S-R425)
Alexa Fluor® 700 Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody (S-R425)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Ly-6G/Ly-6C (Gr-1) |
| Synonyms | Lymphocyte antigen 6G; Ly-6G.1; Ly6g |
| Location | Cell membrane |
| Accession | P35461 |
| Clone Number | S-R425 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
Ly-6G/Ly-6C (Gr-1) is a 21-25 kD myeloid differentiation antigen that is a glycosylphosphatidylinositol (GPI)-anchored protein expressed on granulocytes and macrophages. In mouse bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation, and it is also transiently expressed on bone marrow cells in the monocyte lineage. This antigen is commonly used as a marker for identifying neutrophils and other myeloid cells, and it plays a role in antitumor immunity through its expression on immature myeloid cells.
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