Flow cytometric analysis of mouse CD8β expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 711™ Rat Anti-Mouse CD8a Antibody and either Alexa Fluor® 700 Mouse IgG1, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Rat Anti-Mouse CD8β Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 700 Rat Anti-Mouse CD8β Antibody (53-5.8)
Alexa Fluor® 700 Rat Anti-Mouse CD8β Antibody (53-5.8)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD8β |
| Synonyms | T-cell surface glycoprotein CD8 beta chain; Lymphocyte antigen 3; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein Lyt-3; Cd8b1; Ly-3; Lyt-3; Lyt3; Cd8b |
| Location | Membrane |
| Accession | P10300 |
| Clone Number | 53-5.8 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD8β is a protein that, along with CD8α, forms the CD8 co - receptor complex on the surface of cytotoxic T cells. This complex plays a crucial role in the immune response by facilitating the interaction between cytotoxic T cells and antigen - presenting cells. CD8β helps stabilize the binding of the T cell receptor to MHC class I molecules on the antigen - presenting cell, thereby enhancing the specificity and efficiency of the T cell - mediated immune response. This interaction is essential for the activation of cytotoxic T cells, which can then target and destroy infected or malignant cells.
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