C57BL/6 mouse BMDMs (bone marrow-derived macrophages) were fixed with 4% PFA, permeabilized with 90% methanol. The cells were then stained with Brilliant Violet 421™ Rat Anti-Mouse CD11b Antibody and either Alexa Fluor® 700 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Rat Anti-Mouse CD63 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 700 Rat Anti-Mouse CD63 Antibody (S-R667)
Alexa Fluor® 700 Rat Anti-Mouse CD63 Antibody (S-R667)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD63 |
| Synonyms | CD63 antigen; Cd63 |
| Location | Secreted, Lysosome, Endosome, Cell membrane |
| Accession | P41731 |
| Clone Number | S-R667 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse BMDM |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD63 is a tetraspanin protein involved in various cellular processes such as cell adhesion, migration, and signal transduction. It is predominantly found on the membranes of late endosomes, multivesicular bodies, and exosomes. As a marker protein of exosomes, CD63 plays a crucial role in cell-to-cell communication, including facilitating the interaction between tumor cells and surrounding cells. Additionally, CD63 is associated with intracellular secretory events in cells like eosinophils, where it is involved in degranulation processes. In some cancers, such as hepatocellular carcinoma, CD63-overexpressing tumor-associated macrophages can drive tumor progression by promoting cancer cell proliferation, migration, and invasion.
Picture
Picture
FC
Flow cytometric analysis of CD63 expression on C57BL/6 mouse BMDMs (bone marrow-derived macrophages). C57BL/6 mouse BMDMs were stained with Brilliant Violet 421™ Rat Anti-Mouse CD11b Antibody and either Alexa Fluor® 700 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Rat Anti-Mouse CD63 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
