Flow cytometric analysis of Human CD294 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 700 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Rat Anti-Human CD294 Antibody (right panel) at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 700 Rat Anti-Human CD294 Antibody (S-2867)
Alexa Fluor® 700 Rat Anti-Human CD294 Antibody (S-2867)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD294 |
| Synonyms | Prostaglandin D2 receptor 2; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G-protein coupled receptor 44; CRTH2; DL1R; GPR44; PTGDR2 |
| Location | Cell membrane |
| Accession | Q9Y5Y4 |
| Clone Number | S-2867 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD294, also known as CRTH2, PTGDR2, or GPR44, is a seven-transmembrane G protein-coupled receptor primarily expressed on Th2 cells, eosinophils, basophils, and type-2 innate lymphoid cells (ILC2). It is involved in mediating the chemotaxis of these cells to sites of allergic inflammation, such as the asthmatic lung, and plays a role in preventing apoptosis in Th2 cells.
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