Flow cytometric analysis of human peripheral blood cells labeling Alexa Fluor® 700 Mouse IgG2b, κ Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 700 Mouse IgG2b, κ Isotype Control (S-5677)
Alexa Fluor® 700 Mouse IgG2b, κ Isotype Control (S-5677)
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$230 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Clone Number | S-5677 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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FC
Flow cytometric analysis of A431 (Human epidermoid carcinoma epithelial cell) labeling Alexa Fluor® 700 Mouse IgG2b, κ Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized human PBMCs (human peripheral blood mononuclear cells) labelling Alexa Fluor® 700 Mouse IgG2b, κ Isotype Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized 293F (Human embryonic kidney epithelial cell) labelling Alexa Fluor® 700 Mouse IgG2b, κ Isotype control Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
