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Alexa Fluor® 700 Mouse Anti-Human Granzyme B Antibody (S-3342)

Alexa Fluor® 700 Mouse Anti-Human Granzyme B Antibody (S-3342)

Catalog Number: S0B8804 Application: ICFCM Reactivity: Human Conjugation: Alexa Fluor 700 Brand: Starter
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$195 USD
$195 USD
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Product Details

Product Specification


Host Mouse
Antigen Granzyme B
Synonyms C11; CTLA-1; Cathepsin G-like 1 (CTSGL1); Cytotoxic T-lymphocyte proteinase 2 (Lymphocyte protease); Fragmentin-2; CGL1; CSPB; CTLA1; GRB; GZMB
Location Secreted
Accession P10144
Clone Number S-3342
Antibody Type Mouse mAb
Isotype IgG1,k
Application ICFCM
Reactivity Hu
Positive Sample Human PBMC
Purification Protein G
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 700
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
ICFCM 5μl per million cells in 100μl volume Hu

Background

Granzyme B is a 32-kDa serine protease stored in the cytolytic granules of cytotoxic T lymphocytes and natural killer cells, whose immune function centers on perforin-assisted translocation into target cells where it directly cleaves and activates pro-apoptotic substrates such as procaspase-3, Bid and PARP, thereby igniting both caspase-dependent and caspase-independent mitochondrial apoptosis pathways to eliminate virus-infected or malignant cells.

Picture

FC

Flow cytometric analysis of human Granzyme B expression in Human PBMC. Human PBMC were fixed with 4% PFA and permeabilized with 0.1% Tween. The cells were stained with Phycoerythrin Mouse Anti-Human CD8 Antibody and either Alexa Fluor® 700 Mouse IgG1, κ Isotype Control (left panel) or Alexa Fluor® 700 Mouse Anti-Human Granzyme B Antibody (right panel) at 5 μl/test Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.