Flow cytometric analysis of Human FcεRIα expression on Human PBMC (peripheral blood mononuclear cells. Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD14 Antibody and either Alexa Fluor® 700 Mouse IgG2b, κ Isotype Control (left panel) or Alexa Fluor® 700 Mouse Anti-Human FcεRIα Antibody (right panel) at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 700 Mouse Anti-Human FcεRIα Antibody (AER-37)
Alexa Fluor® 700 Mouse Anti-Human FcεRIα Antibody (AER-37)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | FcεRIα |
| Synonyms | FceRIa, FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor |
| Location | Cell membrane |
| Accession | P12319 |
| Clone Number | AER-37 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
FcεRIα is a single-pass transmembrane glycoprotein of the immunoglobulin superfamily that forms the ligand-binding backbone of the tetrameric high-affinity IgE receptor (FcεRI) expressed on mast cells and basophils; its extracellular region folds into two Ig-like domains that clamp the Fc portion of IgE with picomolar affinity, positioning the constant heavy-chain ε2-3 interface for optimal recognition, while a short cytoplasmic tail lacks signaling motifs but critically nucleates the entire receptor complex by juxtamembrane interactions with the four-helix FcεRIβ subunit and the disulfide-linked FcRγ homodimer, thereby dictating the architecture of the transmembrane three-helix bundle that propagates ITAM-based phosphorylation cascades upon IgE/allergen crosslinking and drives immediate degranulation, cytokine release and the clinical manifestations of type I hypersensitivity.
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