Flow cytometric analysis of CD57 expression on human peripheral blood cells. Human peripheral blood cells were stained with PE Mouse Anti-Human CD56 Antibody and either Alexa Fluor® 700 Mouse IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Mouse Anti-Human CD57 Antibody (right panel) at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 700 Mouse Anti-Human CD57 Antibody (S-3011)
Alexa Fluor® 700 Mouse Anti-Human CD57 Antibody (S-3011)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD57 |
| Synonyms | HNK-1; Leu-7 |
| Location | Cell membrane |
| Clone Number | S-3011 |
| Antibody Type | Mouse mAb |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human peripheral blood cells |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD57 (also designated HNK-1 or Leu-7) is not a protein per se, but a carbohydrate epitope synthesized by the enzymatic action of beta-1,3-glucuronyltransferase 1 (B3GAT1), a type-II membrane protein encoded by the B3GAT1 gene on chromosome 11q25; this enzyme transfers glucuronic acid to terminal N-acetyllactosamine to form the sulfated CD57 determinant on a variety of cell-surface proteins, lipids and chondroitin sulfate proteoglycans, conferring the antigen that marks subsets of terminally differentiated natural killer and CD8+ cytotoxic T lymphocytes, as well as certain neural and muscle cells, and is implicated in neural cell adhesion and the delineation of late-stage lymphocyte maturation.
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