Alexa Fluor® 700 Mouse Anti-Human CD45RA Antibody (HI100)
Alexa Fluor® 700 Mouse Anti-Human CD45RA Antibody (HI100)
Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD45 |
| Synonyms | Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); T200; CD45; PTPRC |
| Location | Cell membrane, Synapse |
| Accession | P08575 |
| Clone Number | HI100 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD45RA is a splice variant of the CD45 protein, encoded by the PTPRC gene, and is a transmembrane glycoprotein with receptor-type protein tyrosine phosphatase activity. It contains the exon 4 in its structure, which distinguishes it from other CD45 isoforms like CD45RO. CD45RA is predominantly expressed on naïve T cells, indicating their potential for activation and differentiation into effector and memory T cells. Its unique extracellular domain, composed of fibronectin type III repeats, is crucial for modulating intracellular signaling pathways, particularly the T-cell receptor (TCR) signaling cascade. The presence of CD45RA on proliferating cells also serves as a marker for tracking clonal expansions in immune responses.
