Flow cytometric analysis of CD42b expression on fresh human peripheral blood cells. Fresh human peripheral blood cells were stained with FITC Mouse Anti-Human CD41a Antibody and either Alexa Fluor® 700 Mouse IgG1, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Mouse Anti-Human CD42b Antibody (right panel) at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 545 (S0B88802), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 700 Mouse Anti-Human CD42b Antibody (S-935-7)
Alexa Fluor® 700 Mouse Anti-Human CD42b Antibody (S-935-7)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD42b |
| Synonyms | Platelet glycoprotein Ib alpha chain; GP-Ib alpha; GPIb-alpha; GPIbA; Glycoprotein Ibalpha; Antigen CD42b-alpha; GP1BA |
| Immunogen | Recombinant Protein |
| Location | Membrane |
| Accession | P07359 |
| Clone Number | S-935-7 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Fresh human peripheral blood cells |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD42b, also known as glycoprotein Ib alpha (GPIbα), is a type I transmembrane glycoprotein expressed on platelets and megakaryocytes. It is a key component of the GPIb-IX-V receptor complex, which plays a critical role in platelet adhesion and thrombus formation by binding to von Willebrand factor (vWF) at sites of vascular injury. CD42b interacts with various molecules, including thrombin, coagulation factors XI and XII, and P-selectin, contributing to hemostasis and thrombosis. Mutations or polymorphisms in CD42b are associated with diseases such as Bernard-Soulier syndrome and platelet-type von Willebrand disease.
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