Flow cytometric analysis of human CD357 expression on PHA activated human PBMCs (human peripheral blood mononuclear cells). Human PBMCs were cultured for 3 days in medium with 10 μg/ml PHA, then the cells were stained with Alexa Fluor® 488 Mouse Anti-Human CD3 Antibody and either Alexa Fluor® 700 Mouse IgG2b, k Isotype Control (left panel) or SDT Alexa Fluor® 700 Mouse Anti-Human CD357 Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 700 Mouse Anti-Human CD357 Antibody (S-3126)
Alexa Fluor® 700 Mouse Anti-Human CD357 Antibody (S-3126)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD357 |
| Synonyms | Tumor necrosis factor receptor superfamily member 18; Activation-inducible TNFR family receptor; Glucocorticoid-induced TNFR-related protein; AITR; GITR; TNFRSF18 |
| Location | Cell membrane |
| Accession | Q9Y5U5 |
| Clone Number | S-3126 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | PHA-treated Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD357, synonymous with GITR (glucocorticoid-induced TNFR-related protein) and TNFRSF18, is a type I transmembrane receptor of the tumor necrosis factor receptor superfamily that is constitutively expressed on CD25⁺CD4⁺ regulatory T cells and up-regulated on activated CD4⁺ and CD8⁺ T cells, NK cells, APCs and many tumors; upon binding its ligand TNFSF18 it recruits TRAF2 and TRAF5, triggering NF-κB and MAPK signaling that enhances effector T-cell proliferation, cytokine production and resistance to suppression by Tregs, positioning CD357 as a promising co-stimulatory target in cancer immunotherapy .
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