Flow cytometric analysis of C57BL/6 mouse splenocytes labeling Alexa Fluor® 700 Human IgG1 Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 700 Human IgG1 Isotype Control (S-R560)
Alexa Fluor® 700 Human IgG1 Isotype Control (S-R560)
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$235 USD
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Product Details
Product Details
Product Specification
| Host | Human |
| Clone Number | S-R560 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG1 |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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FC
Flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast) labeling Alexa Fluor® 700 Human IgG1 Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized C57BL/6 mouse splenocytes labelling Alexa Fluor® 700 Human IgG1 Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling Alexa Fluor® 700 Human IgG1 Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
