Flow cytometric analysis of Mouse CD80 expression on LPS treated C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes treated with LPS (500ng/ml, 3d) were stained with either Alexa Fluor® 700 Armenian hamster IgG Isotype Control (Left panel) or SDT Alexa Fluor® 700 Rat Anti-Mouse CD80 antibody at 1.25 μl/test (Right panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 700 Armenian hamster Anti-Mouse CD80 Antibody (S-R520)
Alexa Fluor® 700 Armenian hamster Anti-Mouse CD80 Antibody (S-R520)
Price:
Regular price
$170 USD
Regular price
Sale price
$170 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | CD80 |
| Synonyms | T-lymphocyte activation antigen CD80; Activation B7-1 antigen (B7); B7; Cd80 |
| Location | Cell membrane |
| Accession | Q00609 |
| Clone Number | S-R520 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | LPS treated C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
CD80，also known as B7-1，is a co - stimulatory molecule expressed on antigen - presenting cells (APCs) such as dendritic cells，macrophages and B cells. It plays a crucial role in the immune response by interacting with CD28 on T cells to provide a co - stimulatory signal necessary for T cell activation，thereby promoting T cell proliferation and differentiation. Additionally，CD80 can also bind to CTLA - 4，an inhibitory receptor on T cells，which acts as a negative regulator of T cell activation. This binding can inhibit the immune response and maintain immune tolerance. Moreover，CD80 - CD28 interaction is involved in the regulation of various immune - related processes，including the formation of immunological synapses and the regulation of cytokine production.
Picture
Picture
FC
