BALB/c mouse splenocytes were stained with SDT Alexa Fluor® 647 Rat IgG2a Isotype Control Antibody (Right panel) at 1/20 dilution (1 μg). Cells without incubation with primary antibody and secondary antibody (Left panel) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software
Alexa Fluor® 647 Rat IgG2a Isotype Control (S-R483)
Alexa Fluor® 647 Rat IgG2a Isotype Control (S-R483)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Clone Number | S-R483 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a |
| Application | FCM, ICFCM |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
Picture
Picture
FC
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) labelling SDT Alexa Fluor® 647 Rat IgG2a Isotype Control Antibody at 1/20 dilution (1 μg) (Red line histogram) compared with unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
