Flow cytometric analysis of Mouse CD335 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with FITC Mouse Anti-Mouse NK1.1 antibody (S0B1825) and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 647 Rat Anti-Mouse CD335 Antibody (right panel) at 5μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse CD335 Antibody (S-R613)
Alexa Fluor® 647 Rat Anti-Mouse CD335 Antibody (S-R613)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD335 |
| Synonyms | Natural cytotoxicity triggering receptor 1; Activating receptor 1 (mAR-1); Lymphocyte antigen 94; Natural killer cell p46-related protein (NK-p46; NKp46; mNKp46); Ly94; Ncr1 |
| Location | Cell membrane |
| Accession | Q8C567 |
| Clone Number | S-R613 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD335, also known as NKp46 or Natural Cytotoxicity Triggering Receptor 1 (NCR1), is a 46 kDa type I transmembrane glycoprotein that belongs to the natural cytotoxicity receptor family. It is primarily expressed on natural killer (NK) cells and plays a crucial role in their activation and cytotoxicity. CD335 can recognize and bind to viral hemagglutinins and heparan sulfate proteoglycans on target cells, triggering NK cell-mediated cytotoxic responses. This receptor is involved in non-MHC-restricted natural cytotoxicity and is essential for the immune system's ability to detect and eliminate infected or transformed cells.
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