Flow cytometric analysis of Mouse CD3ε expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD19 Antibody and either Alexa Fluor® 647 Rat IgG2a Isotype Control (left panel) or SDT Alexa Fluor® 647 Rat Anti-Mouse CD3ε Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse CD3ε Antibody (KT3)
Alexa Fluor® 647 Rat Anti-Mouse CD3ε Antibody (KT3)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD3ε |
| Synonyms | T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; Cd3e |
| Location | Cell membrane |
| Accession | P22646 |
| Clone Number | KT3 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a |
| Isotype Control | S0B8044 |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD3ε protein, also known as CD3E, is a crucial component of the T-cell receptor (TCR) complex, which is essential for T-cell activation and function. It is a single-pass type I membrane glycoprotein that contains one Ig-like domain and one immunoreceptor tyrosine-based activation motif (ITAM) domain. CD3ε, along with CD3γ, CD3δ, and CD3ζ, forms the TCR-CD3 complex, which plays a vital role in coupling antigen recognition to intracellular signal-transduction pathways. This protein is essential for the proper development of T-cells and is involved in the internalization and downregulation of the TCR-CD3 complex on the cell surface. Defects in the CD3ε gene can lead to severe immunodeficiency and have been linked to a susceptibility to type I diabetes in women.
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