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Alexa Fluor® 647 Rat Anti-Mouse CD252 Antibody (S-R735)

Alexa Fluor® 647 Rat Anti-Mouse CD252 Antibody (S-R735)

Catalog Number: S0B8363 Application: FCM Reactivity: Mouse Conjugation: Alexa Fluor 647 Brand: Starter
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$170 USD
$170 USD
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Product Details

Product Specification


Host Rat
Antigen CD252
Synonyms Tumor necrosis factor ligand superfamily member 4; OX40 ligand (OX40L); Ox40l; Txgp1l; Tnfsf4
Location Membrane
Accession P43488
Clone Number S-R735
Antibody Type Rat mAb
Isotype IgG2b,k
Application FCM
Reactivity Ms
Positive Sample stimulated mouse splenocytes B cells
Purification Protein G
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 647
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1.25μl per million cells in 100μl volume Ms

Background

CD252, also known as OX40 ligand (OX40L), is a membrane-bound cytokine of the tumor necrosis factor superfamily expressed on activated antigen-presenting cells that engages its receptor OX40 (CD134) on T cells to deliver a potent costimulatory signal driving T-cell proliferation, survival and effector cytokine production while also participating in B-cell/T-cell cross-talk and being exploited as a therapeutic target in cancer immunotherapy and autoimmune disease modulation.

Picture

FC

Flow cytometric analysis of mouse CD252 expression on stimulated mouse splenocytes B cells. Mouse splenocytes B cells were stimulated (3 days) in culture with Purified Anti-Mouse CD40 antibody and Goat Anti-Mouse IgM. The cells were then stained with either Alexa Fluor® 647 Rat IgG2b, κ Isotype Control or SDT Alexa Fluor® 647 Rat Anti-Mouse CD252 Antibody at 1.25 μl/test Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.