Flow cytometric analysis of Mouse CD25 (IL-2Rα) expression on ConA-treated BALB/c mouse splenocytes. BALB/c mouse splenocytes treated 72h with ConA (right panel) or unstimulated (left Panel) were stained with Brilliant Violet 421™ Rat Anti-Mouse CD4 antibody and SDT Alexa Fluor® 647 Rat Anti-Mouse CD25 (IL-2Rα) antibody at 2μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse CD25 (IL-2Rα) Antibody (PC-61.5.3)
Alexa Fluor® 647 Rat Anti-Mouse CD25 (IL-2Rα) Antibody (PC-61.5.3)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD25 |
| Synonyms | Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; Il2r; Il2ra |
| Location | Membrane |
| Accession | P01590 |
| Clone Number | PC-61.5.3 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,λ |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | ConA-treated Balb/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD25, also known as the interleukin-2 receptor alpha chain (IL2RA), is a type I transmembrane glycoprotein that plays a crucial role in the immune system. It is primarily expressed on the surface of activated T cells and regulatory T cells (Tregs). CD25 is a key component of the high-affinity IL-2 receptor complex, which also includes the IL-2 receptor beta and gamma chains. By itself, CD25 can bind IL-2 with low affinity, but when combined with the beta and gamma chains, it forms a high-affinity receptor that initiates intracellular signaling pathways such as MAPK and JAK/STAT. This receptor complex is essential for the proliferation and activation of T cells, as well as the regulation of immune tolerance by controlling the activity of Tregs. Mutations in the IL2RA gene can lead to deficiencies in IL-2 receptor function, highlighting its importance in maintaining immune homeostasis.
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