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Alexa Fluor® 647 Rat Anti-Mouse CD23 Antibody (S-R672)

Alexa Fluor® 647 Rat Anti-Mouse CD23 Antibody (S-R672)

Catalog Number: S0B8122 Application: FCM Reactivity: Mouse Conjugation: Alexa Fluor® 647 Brand: Starter
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Product Details

Product Specification


Host Rat
Antigen CD23
Synonyms Low affinity immunoglobulin epsilon Fc receptor; Fc-epsilon-RII; Lymphocyte IgE receptor; Fcer2a; Fcer2
Location Secreted, Cell membrane
Accession P20693
Clone Number S-R672
Antibody Type Rat mAb
Isotype IgG2a,k
Application FCM
Reactivity Ms
Positive Sample BALB/c mouse splenocytes
Purification Protein G
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 647
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Ms

Background

CD23, also known as the low-affinity IgE receptor (FcεRII), is a 45 kDa type II integral membrane glycoprotein that plays a crucial role in regulating IgE production and B-cell differentiation. It is expressed on various immune cells, including mature B cells, monocytes, eosinophils, follicular dendritic cells, and platelets. CD23 exists in two isoforms, CD23a and CD23b, which differ in their amino acid sequences and functions. The molecule can also be cleaved into a soluble form (sCD23) that retains IgE-binding capabilities.

Picture

FC

Flow cytometric analysis of Mouse CD23 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD19 Antibody and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Left panel) or SDT Alexa Fluor® 647 Rat Anti-Mouse CD23 Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.