Flow cytometric analysis of mouse CD2 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD19 and either Alexa Fluor® 647 Mouse IgG2b, k Isotype Control (left panel) or SDT Alexa Fluor® 647 Rat Anti-Mouse CD2 Antibody (right panel) at 0.5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse CD2 Antibody (RM2-5)
Alexa Fluor® 647 Rat Anti-Mouse CD2 Antibody (RM2-5)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD2 |
| Synonyms | T-cell surface antigen CD2; LFA-2; LFA-3 receptor; Lymphocyte antigen 37 (Ly-37); T-cell surface antigen T11/Leu-5; Ly-37; Cd2 |
| Location | Cell membrane |
| Accession | P08920 |
| Clone Number | RM2-5 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Isotype Control | S0B8039 |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 0.25μg per million cells in 100μl volume | Ms |
Background
CD2, also known as the T-cell rosette receptor or LFA-2, is a type I membrane protein predominantly expressed on thymocytes and mature T cells. It functions as an adhesion molecule that binds to CD58 (LFA-3) on antigen-presenting cells (APCs), facilitating T-cell receptor (TCR) binding and signal transduction, which is crucial for T-cell activation and immune responses.
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