Flow cytometric analysis of Mouse CD19 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes cells were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 antibody and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 647 Rat Anti-Mouse CD19 antibody (right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse CD19 Antibody (1D3)
Alexa Fluor® 647 Rat Anti-Mouse CD19 Antibody (1D3)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD19 |
| Location | Cell membrane |
| Accession | P25918 |
| Clone Number | 1D3 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a |
| Isotype Control | Alexa Fluor® 647 Rat IgG2a Isotype Control (S-R483) |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
CD19 is a 95 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily and is expressed on the surface of B lymphocytes and follicular dendritic cells. It plays a crucial role in B cell activation and humoral immune responses by forming a complex with CD21, CD81, and CD225. This complex reduces the threshold for B cell receptor (BCR) activation, enhancing downstream signaling pathways and facilitating B cell responses to antigens. CD19 is also a biomarker for B cell development and lymphoma diagnosis, and it is targeted in immunotherapies for diseases like leukemia.
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