Flow cytometric analysis of human Podoplanin expression on NCCIT (Human pluripotent embryonic carcinoma epithelial cell). Cells from the NCCIT were stained with either Alexa Fluor® 647 Rat IgG2a, λ Isotype Control (black line histogram) or SDT Alexa Fluor® 647 Rat Anti-Human Podoplanin Antibody (red line histogram) at 1.25μl/test, cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Human Podoplanin Antibody (S-2796)
Alexa Fluor® 647 Rat Anti-Human Podoplanin Antibody (S-2796)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Podoplanin |
| Synonyms | Podoplanin; Aggrus; Glycoprotein 36 (Gp36); PA2.26 antigen; T1-alpha (T1A); GP36; PDPN |
| Location | Membrane |
| Accession | Q86YL7 |
| Clone Number | S-2796 |
| Antibody Type | Rat mAb |
| Isotype Control | S0B8044 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | NCCIT |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
Podoplanin is a small mucin-type O-glycosylated type-I transmembrane protein of 162 amino acids, highly expressed on lymphatic endothelium, alveolar type I cells, tumor cells and brain neurons, where its extracellular domain—bearing the platelet-aggregation-stimulating PLAG motif and sialylated O-glycans—engages CLEC-2 on platelets to trigger platelet activation, binds CD44 and galectin-8 to promote directional cell migration and invadopodia maturation, associates with ERM proteins (ezrin-radixin-moesin) to reorganize the actin cytoskeleton and drive epithelial-mesenchymal transition, and is indispensable for developmental separation of blood and lymphatic vessels, normal alveolus formation, lymphatic vessel integrity and hippocampus-dependent synaptic plasticity and memory, while its overexpression in cancers fosters invasion, metastasis and thrombotic risk, making it a key diagnostic and therapeutic target.
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