Flow cytometric analysis of mouse CD272 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Pacific Blue™ Rat Anti-Mouse CD3 Antibody and either Alexa Fluor® 647 Rabbit IgG Isotype Control (left panel) or SDT Alexa Fluor® 647 Rabbit Anti-Mouse/Rat BTLA/CD272 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Rabbit Anti-Mouse/Rat BTLA/CD272 Antibody (S-742-13-2)
Alexa Fluor® 647 Rabbit Anti-Mouse/Rat BTLA/CD272 Antibody (S-742-13-2)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BTLA (CD272) |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q7TSA3、Q6PNM1 |
| Clone Number | S-742-13-2 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Isotype Control | S0B8021 |
| Application | FCM |
| Reactivity | Ms, Rt |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms, Rt |
Background
BTLA (CD272) is a ~34–43 kDa type-I transmembrane glycoprotein of the CD28/CTLA-4 Ig superfamily that is constitutively expressed on T, B, NK and dendritic cells, where it delivers inhibitory signals through two ITIMs and an ITSM in its cytoplasmic tail after binding its ligand HVEM (CD270), thereby attenuating T- and B-cell proliferation, cytokine production and cytolytic activity, recruiting SHP-1/SHP-2 phosphatases to maintain immune homeostasis and self-tolerance, and acting in concert with PD-1 and CTLA-4 as an immune-checkpoint axis whose down-regulation on tumor or autoreactive lymphocytes promotes immune evasion or autoimmune pathologies such as SLE.
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