Flow cytometric analysis of Phospho-Stat5 (Tyr694) expression on IL-2-treated Human PBMC. Human PBMC (peripheral blood mononuclear cells) treated 15min with IL-2 were stained with either Alexa Fluor® 647 Rabbit IgG Isotype Control (Left panel) or SDT Phospho-Stat5 (Tyr694) Recombinant Rabbit mAb (A647 Conjugate) (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Rabbit Anti-Human Phospho-Stat5 (Tyr694) Antibody (S-1183-58)
Alexa Fluor® 647 Rabbit Anti-Human Phospho-Stat5 (Tyr694) Antibody (S-1183-58)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Stat5 (Tyr694) |
| Synonyms | Signal transducer and activator of transcription 5A; STAT5; STAT5A |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P42229 |
| Clone Number | S-1183-58 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | IL-2-treated Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Hu |
Background
Phospho-Stat5 (Tyr694) is the transcription factor Signal Transducer and Activator of Transcription 5 that has undergone phosphorylation on tyrosine residue 694 within its C-terminal SH2 domain; this single, cytokine-activated modification induces Stat5 dimerization, nuclear translocation, and sequence-specific DNA binding to GAS elements, thereby driving transcription of genes controlling proliferation, survival, erythropoiesis, lactation, and immune regulation, with aberrant pY694-Stat5 signaling being a hallmark of several hematologic malignancies and solid tumors where it sustains oncogenic gene expression programs.
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