Flow cytometric analysis of Human CD44V6 expression on A431 cells. Cells from the A431 (Human epidermoid carcinoma epithelial cell, right) or Raji (Human Burkitt's lymphoma B lymphocyte, left) was stained with either Alexa Fluor® 647 Rabbit IgG Isotype Control (black line histogram) or SDT Alexa Fluor® 647 Rabbit Anti-Human CD44v6 Antibody (red line histogram) at 1.25 μl/test, cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Rabbit Anti-Human CD44v6 Antibody (S-944-13)
Alexa Fluor® 647 Rabbit Anti-Human CD44v6 Antibody (S-944-13)
Price:
Regular price
$85 USD
Regular price
Sale price
$85 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD44v6 |
| Synonyms | CD44 antigen; CDw44; Epican; Extracellular matrix receptor III (ECMR-III); GP90 lymphocyte homing/adhesion receptor; HUTCH-I; LHR; MDU2; MDU3; MIC4 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P16070 |
| Clone Number | S-944-13 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Isotype Control | S0B8021 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | A431 |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD44 variable alternative exon 6 (CD44 V6) is a variant isoform of the CD44 gene resulting from alternative splicing of RNA. CD44 is a cell adhesion molecule that is present in various cell types and plays crucial roles in biological processes such as cell migration, proliferation, and differentiation. The CD44 V6 isoform specifically, has been implicated in tumor development and metastasis. Research has shown that CD44v6 is expressed by all colorectal cancer stem cells (CR-CSCs). The expression of CD44v6 is essential for the migration and formation of metastatic tumors, achieved through the activation of the WNT-β-catenin signaling pathway. Propelling evidence recommends that CD44v6 compared to CD44 has a more specific expression in CSCs and may have a higher diagnostic value.
Picture
Picture
FC
