Flow cytometric analysis of CD14 expression on human peripheral blood cells. Human peripheral blood cells were stained with Alexa Fluor® 647 Rabbit IgG Isotype Control (left panel) or SDT Alexa Fluor® 647 Rabbit Anti-Human CD14 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Rabbit Anti-Human CD14 Antibody (S-395-8)
Alexa Fluor® 647 Rabbit Anti-Human CD14 Antibody (S-395-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD14 |
| Synonyms | Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein |
| Immunogen | Recombinant Protein |
| Location | Secreted, Golgi Apparatus, Cell membrane |
| Accession | P08571 |
| Clone Number | S-395-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Isotype Control | S0B8021 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human Peripheral Blood cells |
| Purification | Protein A |
| Concentration | 0.1 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 2μl per million cells in 100μl volume | Hu |
Background
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein primarily expressed on monocytes, macrophages, and some granulocytes. It functions as a pattern recognition receptor (PRR) and plays a crucial role in the innate immune response by recognizing bacterial lipopolysaccharides (LPS), pathogen-associated molecular patterns (PAMPs), and damage-associated molecular patterns (DAMPs). CD14 exists in two forms: membrane-bound CD14 (mCD14) and soluble CD14 (sCD14), which can be secreted by activated cells. It acts as a co-receptor for Toll-like receptor 4 (TLR4) and enhances the immune response to LPS by facilitating its delivery to the TLR4-MD2 complex. Additionally, CD14 is involved in TLR4-independent signaling pathways and contributes to tissue homeostasis by promoting the clearance of apoptotic cells.
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