Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cells) labeling Alexa Fluor® 647 Mouse IgG1, κ Isotype Control Antibody at 1/20 dilution (1 μg). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Clone Number | S-844-79 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM, ICFCM |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized Human PBMC (human peripheral blood mononuclear cells) labeling Alexa Fluor® 647 Mouse IgG1, κ Isotype Control Antibody at 1/20 dilution (1 μg). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
