Flow cytometric analysis of Rat CD90 expression on Lewis Rat splenocytes. Lewis Rat splenocytes were stained with Phycoerythrin Mouse Anti-Rat CD3 Antibody and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Left panel) or SDT Alexa Fluor® 647 Mouse Anti-Rat CD90 Antibody (Right panel) at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Rat CD90 Antibody (S-R687)
Alexa Fluor® 647 Mouse Anti-Rat CD90 Antibody (S-R687)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD90 |
| Synonyms | Thy-1 membrane glycoprotein; Thy-1 antigen; Thy-1; Thy1 |
| Location | Cell membrane |
| Accession | P01830 |
| Clone Number | S-R687 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | Lewis Rat splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Rt |
Background
CD90, also known as Thy-1, is a 25-35 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein and a member of the immunoglobulin superfamily. It is expressed on various cell types, including T cells, neuroglial cells, fibroblasts, and some endothelial cells. CD90 plays important roles in cell-cell and cell-matrix interactions, cell adhesion, and differentiation. It is also a marker for several types of stem cells, such as mesenchymal stem cells (MSCs) and hematopoietic stem cells. Additionally, CD90 is involved in integrin-mediated signaling pathways and can regulate cell migration and stiffness sensing.
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