Flow cytometric analysis of CD31 expression on SD rat splenocytes. SD rat splenocytes were stained with PE Mouse Anti-Rat CD45RA Antibody and either Alexa Fluor® 647 Mouse IgG1, k Isotype Control (left panel) or SDT Alexa Fluor® 647 Mouse Anti-Rat CD31 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 545 (S0B88802), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Rat CD31 Antibody (TLD-3A12)
Alexa Fluor® 647 Mouse Anti-Rat CD31 Antibody (TLD-3A12)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD31 |
| Synonyms | Platelet endothelial cell adhesion molecule; PECAM-1; Pecam; Pecam1 |
| Location | Cell membrane |
| Accession | Q3SWT0 |
| Clone Number | TLD-3A12 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Isotype Control | S0B5964 |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | SD rat splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Rt |
Background
CD31 (PECAM-1) is a 130 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily, expressed constitutively on endothelial cells, platelets, monocytes, neutrophils and some T cells, where it localizes to intercellular junctions and functions as a mechanosensitive adhesion and signaling hub that orchestrates leukocyte transendothelial migration through homophilic CD31-CD31 interactions and heterophilic binding to partners such as CD177 and integrins, while simultaneously regulating endothelial permeability, angiogenic sprouting and vascular integrity via Src- and PI3K-mediated phosphorylation of its short cytoplasmic tail, making it a key biomarker for microvessel density in tumors and a checkpoint regulator of monocyte FcγR responses.
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