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Alexa Fluor® 647 Mouse Anti-Mouse XCR1 Antibody (S-R601)

Alexa Fluor® 647 Mouse Anti-Mouse XCR1 Antibody (S-R601)

Catalog Number: S0B8483 Application: FCM Reactivity: Ms Conjugation: Alexa Fluor® 647 Brand: Starter
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$70 USD
$70 USD
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Product Details

Product Specification


Host Mouse
Antigen XCR1
Synonyms Chemokine XC receptor 1; Lymphotactin receptor; SCM1 receptor; XC chemokine receptor 1; mXCR1; Ccxcr1; Xcr1
Location Cell membrane
Accession Q9R0M1
Clone Number S-R601
Antibody Type Mouse mAb
Isotype IgG2b,k
Application FCM
Reactivity Ms
Positive Sample positive selection of CD8+ cells from C57BL/6 mouse splenocytes
Purification Protein A
Concentration 0.1 mg/ml
Conjugation Alexa Fluor® 647
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1μl per million cells in 100μl volume Ms

Background

XCR1 is a conserved, 37-kDa, seven-transmembrane, G-protein-coupled receptor selectively expressed on CD8α⁺ and CD103⁺ conventional dendritic cells; its only ligand, XCL1, is secreted by activated CD8⁺ T and NK cells to form a feed-forward loop that efficiently recruits antigen-bearing dendritic cells into draining lymph nodes, licenses cross-presentation, and amplifies cytotoxic immune responses against viruses and tumors, while genetic or antibody blockade of XCR1 impairs viral clearance, vaccine efficacy, and checkpoint-blockade-mediated anti-tumor immunity.

Picture

FC

Flow cytometric analysis of Mouse XCR1 expression on positive selection of CD8+ cells from C57BL/6 mouse splenocytes. CD8+ cells were stained with Pacific Blue™ Rat Anti-Mouse CD8 (S0B5067) antibody, PE Rat Anti-Mouse CD11c antibody and either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Left panel) or SDT Alexa Fluor® 647 Mouse Anti- Mouse XCR1 antibody (Right panel) at 1 μl/test. Data shown was gated on the CD11c+ population. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.