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Alexa Fluor® 647 Mouse Anti-Mouse H-2Kd Antibody (SF1-1.1)

Alexa Fluor® 647 Mouse Anti-Mouse H-2Kd Antibody (SF1-1.1)

Catalog Number: S0B8645 Application: FCM Reactivity: Ms Conjugation: Alexa Fluor® 647 Brand: Starter
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$123 USD
$123 USD
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Product Details

Product Specification


Host Mouse
Antigen H-2Kd
Synonyms H-2 class I histocompatibility antigen, K-D alpha chain; H-2K(D); H2-K; H2-K1
Location Membrane
Accession P01902
Clone Number SF1-1.1
Antibody Type Mouse mAb
Isotype IgG2a,k
Application FCM
Reactivity Ms
Positive Sample BALB/c mouse splenocytes
Purification Protein A
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 647
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Ms

Background

H-2Kd is a murine MHC class I molecule expressed by BALB/c mice that presents endogenous 9-mer peptides—anchored by Tyr at position 2 and a hydrophobic residue at position 9—to CD8⁺ cytotoxic T cells, thereby initiating adaptive immune responses; its 2.6-Å crystal structure reveals a peptide-binding groove with five deep pockets (A–F) that impose an allele-specific motif, and it pairs non-covalently with β2-microglobulin for stability.

Picture

FC

Flow cytometric analysis of Mouse H-2Kd expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Mouse Anti-Mouse H-2Kd Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.